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Yokogawa Electric
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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for extracting live blastoderm cells from embryos of annual killifish
doi: 10.1016/j.xpro.2023.102344
Figure Lengend Snippet: Yield of A. nigripinnis blastoderm cells (deep embryonic and EVL cells) extracted at early post epiboly stages (A) Quantification of cell viability after extraction using the Neubauer chamber. Live cells appear green as they retain Calcein-AM fluorophore. Large cells are EVL cells and small are deep cells. (B) Exemplary images of a quadrant of the Neubauer chamber (left) and enlarged view (right) illustrate how to recognize EVL and deep cells and how to distinguish between live cells (those that retain Calcein-AM – green arrows) and dead cells (without fluorescence – red arrow). Images were acquired using a Nikon Ti2 eclipse inverted microscope with a 10× air objective with 0.30 NA. Scale bar: 100 μm. (C) Comparison of cell size allows distinguishing EVL (diameter above 10 μm) from deep cells (about 5 μm in diameter). Live cells also typically display cellular activities such as presence of cellular extrusion. Images were acquired using a Nikon Ti2 eclipse inverted microscope with a 40× air objective with 0.95 NA. Scale bar: 20 μm.
Article Snippet: Images were acquired using a
Techniques: Extraction, Fluorescence, Inverted Microscopy, Comparison
Journal: STAR Protocols
Article Title: Protocol for extracting live blastoderm cells from embryos of annual killifish
doi: 10.1016/j.xpro.2023.102344
Figure Lengend Snippet:
Article Snippet: Images were acquired using a
Techniques: Recombinant, Inverted Microscopy, Clinical Proteomics, Cell Culture, Sterility, Transferring